ocn  (TaKaRa)

Journal: bioRxiv

Article Title: Interleukin-3 as a Potential Bone Anabolic Agent in treating Postmenopausal Osteoporosis

doi: 10.1101/2025.07.12.664485

Figure Lengend Snippet: IL-3 alters OVX-induced changes in osteoclasts- and osteoblasts-specific gene expression. Marrow free bones from IL-3 treated animals from both Prophylactic and Therapeutic treatment strategy, as indicated, were subjected to mRNA expression profile and ( A, B ) Osteoclast-specific markers: integrinβ3, Dc-stamp, and Nfatc1 , Ctsk, Tnfrsf11a(RANK), Mmp9, Trap, and Ctr. (B, D) Osteoblast-specific markers: Runx2, Osx, Opn, Alp, Bmp2, Bmp4, Ocn, Bmp6, and Col1 were assessed by qPCR. Serum ( E, G ) CTX and ( F, H ) OCN were also measured by ELISA in both the strategies. Data is presented as mean ± SEM with n=3 mice/group for qPCR, and n=8 mice/group for ELISA.

Article Snippet: The serum level of CTX-I (CUSABIO; CSB-E12782m) and OCN (CUSABIO ; CSB-E06917m) was assessed by ELISA according to manufacturers’ instructions.

Techniques: Gene Expression, Expressing, Enzyme-linked Immunosorbent Assay

Journal: Experimental & Molecular Medicine

Article Title: Hypoxia-inducible factor-2α mediates senescence-associated intrinsic mechanisms of age-related bone loss

doi: 10.1038/s12276-021-00594-y

Figure Lengend Snippet: a Detection of the indicated mRNAs in the long bones of 1.5-, 4-, and 20-month-old mice. The levels of mRNAs encoded by Hif-2α , osteoblast marker genes ( Ocn and Runx2 ), an osteoclast marker gene ( Trap ) and senescence marker genes ( p16 and p21 ) were determined by RT-PCR and quantified by qRT-PCR ( n ≥ 4). b The protein levels of HIF-2α extracted from mice of the indicated age determined by western blot analysis (left panel) and quantified by a CS analyzer 4 (right panel) ( n = 5). c ELISA-based measurements of the serum concentrations of OCN ( n = 5) and CTX-1 ( n = 5) in 1.5-, 4-, 12- and 20-month-old mice. d, e Immunostaining of osteoblasts ( d ) and osteoclasts ( e ) from the bone tissues of young (4-month-old) and old (12-month-old) mice for HIF-2α. The dotted lines indicate osteoblasts, and the arrowheads indicate osteoclasts. Scale bar: 25 μm. Quantification of HIF-2α-positive cells is shown in the right panel ( n = 8). M = month. The values are presented as the means ± SDs (* P < 0.05, ** P < 0.01, and *** P < 0.005).

Article Snippet: Serum OCN and CTX-1 levels were measured using a mouse Gla-OCN High Sensitive EIA Kit (MK127; TaKaRa Bio) and CTX-1 EIA Kit (NBP2-69074; Novus Biologicals).

Techniques: Marker, Reverse Transcription Polymerase Chain Reaction, Quantitative RT-PCR, Western Blot, Enzyme-linked Immunosorbent Assay, Immunostaining

Journal: Experimental & Molecular Medicine

Article Title: Hypoxia-inducible factor-2α mediates senescence-associated intrinsic mechanisms of age-related bone loss

doi: 10.1038/s12276-021-00594-y

Figure Lengend Snippet: a , b Doxorubicin-induced inhibition of osteoblast differentiation. Primary calvarial preosteoblasts from newborn mice were cultured in osteogenic differentiation medium (DM) containing 50 μg/ml L-AA and 5 mM β-GP for 7 days. Before harvesting cells on day 14, 0.01 μg/ml doxorubicin was added to the differentiation medium for 1, 3, or 5 days. Representative images of alkaline phosphatase (ALP) and alizarin red S (ARS) staining in primary calvarial preosteoblasts cultured in control medium (CM) or differentiation medium in the absence or presence of 0.01 μg/ml doxorubicin are shown ( a ). The expression levels of Hif-2α, Ocn, Runx2, p16 , and p21 were analyzed by qRT-PCR ( n = 5; b ). c Quantification of mRNA levels by qRT-PCR. The transcript levels of the indicated genes were measured in primary calvarial preosteoblasts infected with Ad-C at an MOI of 800 or Ad -Hif-2α at the indicated MOI ( n ≥ 4). d The mRNA levels of Hif-2α, Ocn, Runx2, p16 , and p21 . Osteoblasts isolated from Hif-2α fl/fl mice were infected with Ad-C or Ad- Cre in the presence of differentiation medium ( n ≥ 4). e , f Effect of HIF-2α inhibition on osteoblast matrix maturation. Doxorubicin-treated osteoblasts were treated with the potent HIF-2α inhibitor TC-S 7009 and incubated for 3 days. The levels of Ocn , Runx2, p16 , and p21 mRNAs were measured by qRT-PCR ( n = 5; e ). Assessment of osteoblast differentiation and mineral deposition by ALP and ARS staining ( f ). The values are presented as the means ± SDs (* P < 0.05, ** P < 0.01, and *** P < 0.005; NS not significant).

Article Snippet: Serum OCN and CTX-1 levels were measured using a mouse Gla-OCN High Sensitive EIA Kit (MK127; TaKaRa Bio) and CTX-1 EIA Kit (NBP2-69074; Novus Biologicals).

Techniques: Inhibition, Cell Culture, Staining, Expressing, Quantitative RT-PCR, Infection, Isolation, Incubation